CCR7 Rabbit pAb - P47774

Core Product Specifications and Parameters

Biological Background: CCR7 Function and Localization

• Encoded by the Ccr7 gene, C-C chemokine receptor type 7 (also known as EBI1, CD197, or MIP-3 beta receptor) is a multi-pass transmembrane protein belonging to the G protein-coupled receptor (GPCR) family.
• CCR7 functions as a high-affinity receptor for the chemokine MIP-3-beta/CCL19, playing a pivotal role in directing immune cell trafficking to secondary lymphoid organs.
• Structurally, CCR7 contains conserved features typical of chemokine receptors, including an extracellular N-terminus, seven transmembrane helices, and an intracellular C-terminus.
• Localized predominantly at the cell membrane, CCR7 is expressed in various lymphocyte subsets and dendritic cells, where it mediates homeostatic and inflammatory homing.
• Key post-translational modifications, such as glycosylation and disulfide bond formation, are critical for receptor stability, ligand binding, and signaling competence.
• Beyond lymphocyte homing, CCR7 signaling influences thymocyte development, regulatory T cell migration, and the establishment of immune tolerance.
• In the context of disease, CCR7 is implicated in cancer metastasis and chronic inflammatory conditions, making it a target of interest for therapeutic and diagnostic applications.
• The protein’s calculated molecular weight is approximately 43 kDa, consistent with the observed band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide spanning residues 211–310 within the intracellular C-terminal domain of mouse CCR7 (NP_031745.2). This region is predicted to be less conserved across species, which may contribute to the reactivity with rat CCR7.
• For western blotting (WB), consider using lysates from lymphoid tissues (e.g., spleen, lymph node) or cell lines known to express CCR7 (e.g., EL4 mouse thymoma cells). Antigen retrieval or enrichment steps may enhance detection due to low endogenous expression.
• The observed band size (~43 kDa) may shift due to glycosylation or other modifications; inclusion of deglycosylation enzymes can aid in validating specificity.
• In ELISA applications, this polyclonal antibody can be used as a capture or detection reagent; pairing with a monoclonal anti-CCR7 antibody may improve assay specificity. Validate the optimal working concentration through checkerboard titration in your sample matrix.
• As with any polyclonal reagent, lot-to-lot consistency should be verified, and cross-adsorption steps may be considered to minimize non-specific binding to other chemokine receptors.

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