CCL7 Rabbit pAb for WB, ELISA - P80098

Core Product Specifications and Parameters

Biological Background: CCL7 Function and Localization

• CCL7, also known as monocyte chemoattractant protein 3 (MCP-3), is a member of the CC chemokine family.
• Acts as a chemotactic factor, attracting monocytes, eosinophils, basophils, and T-cells to sites of inflammation or infection via binding to chemokine receptors CCR1, CCR2, CCR3, and CCR5. Related references: PMID:9364936 PMID:10477718
• Plays a role in monocyte homeostasis by interacting with CCR2 to release monocytes from bone marrow; these monocytes can differentiate into macrophages or dendritic cells.
• Through CCR1, contributes to macrophage polarization via NF-kappa-B activation, leading to the release of inflammatory factors. Related references: PMID:38417634
• In the trigeminal ganglion, activates ERK signaling via CCR2 and CCR3, enhancing neuronal excitability and contributing to trigeminal neuropathic pain.
• Modulates the early immune response in the skin, preventing pathogen dissemination while maintaining cutaneous immune control.
• Secreted protein with a molecular weight of approximately 11 kDa; undergoes post-translational modifications including disulfide bond formation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length mature CCL7 sequence (amino acids 1-99), suggesting broad epitope coverage from this polyclonal antibody.
• For Western blotting, note that CCL7 has a predicted size of ~11 kDa; consider using high-percentage gels and optimized transfer conditions for small proteins.
• In ELISA, the polyclonal nature may offer high sensitivity; consider pairing with a monoclonal capture antibody for sandwich ELISA development.
• Cross-reactivity with rat CCL7 enables potential use in rodent inflammation models; however, validate the antibody in the relevant sample system.
• As a secreted chemokine, ensure that sample preparations (e.g., serum, conditioned media, tissue lysates) appropriately preserve CCL7 expression for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop sourcing partner for IVD raw materials, supporting manufacturers and research labs from development to commercialization. This CCL7 polyclonal antibody exemplifies our portfolio in immune-regulation and inflammation targets. Explore our validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing solutions to streamline your diagnostic assembly and research workflows.