CCAR2/DBC1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N163

Core Product Specifications and Parameters

Biological Background: CCAR2/DBC1 Function and Localization

• Acts as a core component of the DBIRD complex, integrating transcript elongation with alternative splicing at (A+T)-rich DNA regions. Related references: PMID:22446626

• Functions as a negative regulator of SIRT1 deacetylase, promoting p53/TP53 acetylation and p53-mediated apoptosis; also inhibits SUV39H1 methyltransferase activity. Related references: PMID:18235501, PMID:18235502, PMID:23352644, PMID:19218236

• Regulates circadian rhythms by modulating NR1D1 and BMAL1 expression, and enhances NR1D1 repressor activity through protein stabilization. Related references: PMID:23398316

• Maintains genomic stability and facilitates DNA damage response upon UV-induced genotoxic stress. Related references: PMID:23398316

• Mediates tumor suppression by stabilizing p53/TP53 via competitive inhibition of MDM2 ubiquitin ligase. Related references: PMID:25732823

• Localizes predominantly to the nucleus but also to the cytoplasm and spindle apparatus, reflecting its roles in transcription and cell division.

• Subject to multiple post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitin-like conjugation, which may regulate its diverse functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-100 amino acids of human CCAR2/DBC1; consider sequence alignment to verify cross-reactivity with mouse and rat orthologs.
• Recommended applications include western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA; validate dilution and detection conditions in your specific sample system.
• Due to its high molecular weight (~102-103 kDa), use appropriate gel percentages and transfer conditions for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents for diagnostic and research applications. This CCAR2/DBC1 polyclonal antibody is part of our one-stop sourcing solution to support your assay development from target discovery to clinical validation. Contact us to discuss customized bulk orders or rare-target raw material sourcing for your oncology or epigenetics projects.