CBX2 Rabbit Monoclonal Antibody for WB & ELISA - Q14781

Core Product Specifications and Parameters

Biological Background: CBX2 Function and Localization

• CBX2 is a core component of the Polycomb repressive complex 1 (PRC1), critical for maintaining the transcriptionally repressed state of numerous developmental genes, including Hox clusters. Related references: PMID:21282530
• It mediates monoubiquitination of histone H2A at Lys-119 (H2AK119ub1), a chromatin mark that heritably alters gene expressibility. Related references: PMID:21282530
• The chromodomain of CBX2 specifically binds to histone H3 trimethylated at Lys-9 (H3K9me3) or Lys-27 (H3K27me3), tethering the PRC1 complex to regions of silent chromatin.
• In embryonic development, CBX2 promotes lineage differentiation of germ layers, contributing to early cell fate decisions.
• CBX2 functions as an activator of NR5A1 expression, playing a pivotal role in sexual development and gonadogenesis. Related references: PMID:19361780
• Subcellularly, CBX2 is localized strictly to the nucleus and associates with chromosomes, consistent with its role in chromatin organization.
• Post-translational modifications, including phosphorylation, methylation, and ubiquitination, are predicted to modulate CBX2 function, while alternative splicing generates functionally distinct isoforms.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide spanning amino acids 433–532 of human CBX2, implying recognition of a linear epitope. It is therefore suitable for Western blotting under denaturing and reducing conditions to detect CBX2 at its predicted molecular weight of ~56 kDa.
• For ELISA applications, consider coating with the immunogen peptide or recombinant CBX2 protein, and optimize antibody dilution and detection conditions experimentally.
• Cross-reactivity with mouse and rat is indicated, but in-species validation is recommended by testing the antibody in relevant cell lines or tissue lysates from these organisms.
• As CBX2 is a nuclear chromatin-associated protein, nuclear extraction or chromatin fractionation may enhance detection sensitivity in certain sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical support. This anti-CBX2 monoclonal antibody exemplifies our commitment to offering high-quality reagents for epigenetic and developmental biology research. Beyond single antibodies, CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist in sourcing rare targets to streamline your assay development from concept to clinic.