CBFB Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - Q13951

Core Product Specifications and Parameters

Biological Background: CBFB Function and Localization

• CBFB is the non-DNA-binding beta subunit of the core-binding factor (CBF) heterodimeric transcription factor complex. It allosterically enhances the DNA-binding affinity of RUNX family proteins (RUNX1, RUNX2, RUNX3).
• The CBF complex recognizes the core consensus sequence 5'-TGTGGT-3' (rarely 5'-TGCGGT-3') in regulatory regions of target genes.
• CBFB/RUNX heterodimers regulate expression of critical genes involved in hematopoiesis and immune function, including T-cell receptor enhancers, LCK, IL3, and GM-CSF.
• During CD8+ T cell development, CBF complexes repress ZBTB7B transcription factor expression by binding to RUNX-binding sites within the ZBTB7B locus, acting as a transcriptional silencer to permit cytotoxic T cell differentiation.
• Following HIV-1 infection, the viral protein Vif hijacks CBFB to assemble a cullin-5-RING E3 ubiquitin ligase complex (ECS), which targets APOBEC3F, APOBEC3G, and to a lesser extent APOBEC3H for ubiquitination and proteasomal degradation, thus counteracting host antiviral defenses. Related references: PMID:22190037 PMID:31792451 PMID:36598981 PMID:37640699
• CBFB localizes predominantly to the nucleus, consistent with its role as a transcriptional coregulator.
• The CBFB gene undergoes alternative splicing and is implicated in chromosomal rearrangements, particularly in acute myeloid leukemia (AML), and is classified as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-182 of human CBFB, covering the conserved heterodimerization domain; therefore the antibody is expected to recognize all major CBFB isoforms.
• For Western blotting, the detected band should appear at approximately 22 kDa; consider using nuclear-enriched lysates and validating with a known positive control.
• For IHC-P and IF/ICC, pilot experiments should optimize antigen retrieval and fixation methods; CBFB nuclear localization may require permeabilization protocols.
• For ELISA, this polyclonal antibody may serve as a capture or detection reagent; pairing with monoclonal antibodies should be evaluated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic assay development. For CBFB-related research on hematological malignancies and immune regulation, we offer optimized detection tools and support rare target raw-material sourcing to accelerate your projects from concept to clinic.