Caveolin-3 Rabbit pAb - P56539

Core Product Specifications and Parameters

Biological Background: Caveolin-3 Function and Localization

• Caveolin-3 (M-caveolin) is a muscle-specific integral membrane protein and the principal structural component of caveolae in striated muscle.
• It functions as a scaffolding protein within caveolar membranes, directly interacting with and regulating G-protein alpha subunits and voltage-gated potassium channels.
• Plays a critical role in sarcolemma repair, enabling rapid resealing of mechanically disrupted membranes in skeletal muscle and cardiomyocytes (By similarity).
• Mediates the recruitment of CAVIN2 and CAVIN3 proteins to caveolae, essential for caveola formation and stability (PubMed:19262564). Related references: PMID:19262564
• Subcellular localization includes the Golgi apparatus membrane, cell membrane, caveola, and sarcolemma.
• Expression is predominantly in muscle tissues, consistent with its role in muscle cell membrane integrity.
• Keywords from UniProt associate CAV3 with cardiomyopathy, limb-girdle muscular dystrophy, and long QT syndrome, highlighting its clinical significance in muscle and cardiac function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human Caveolin-3, ensuring epitope recognition across species with high sequence homology (human, mouse, rat).
• For Western blot (WB), the predicted molecular weight of Caveolin-3 is approximately 17 kDa; consider using muscle tissue or cell lysates that express caveolin-3.
• Validate antibody specificity in your sample system by including appropriate positive (e.g., skeletal muscle) and negative controls.
• For ELISA, the synthetic peptide immunogen may serve as a coating antigen; optimization of coating conditions and antibody dilutions is recommended.
• Primary antibody concentrations should be empirically determined for each application; starting dilutions for WB are often in the range of 1:500–1:2000, but must be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and laboratories worldwide. As a one-stop sourcing partner, we offer validated antibody pairs, high-performance rabbit polyclonals like this anti-Caveolin-3 antibody, and bulk ancillary reagents to support muscle-related biomarker research and assay development. Our technical team assists with rare target sourcing and custom material evaluation, ensuring your project progresses from concept to clinic efficiently.