Cathepsin G Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P08311

Core Product Specifications and Parameters

Biological Background: Cathepsin G Function and Localization

• Cathepsin G is a serine protease with trypsin- and chymotrypsin-like specificity, preferentially cleaving after Phe, Tyr, Trp, and Leu residues. Related references: PMID:29652924 PMID:8194606
• Displays antibacterial activity against both Gram-negative and Gram-positive bacteria, independent of its protease activity. Related references: PMID:2116408 PMID:2117044
• Required for recruitment and activation of platelets via the F2RL3/PAR4 platelet receptor. Related references: PMID:10702240 PMID:3390156
• Binds reversibly to B cells, CD4+ and CD8+ T cells, and NK cells, enhancing immune cell function. Related references: PMID:7842483 PMID:9000539 PMID:9536127
• Localizes to multiple subcellular compartments including cytoplasmic granules, lysosomes, cell membrane, and nucleus, reflecting its diverse functions.
• Expressed in neutrophils and B cells at the protein level. Related references: PMID:3799965 PMID:15100291
• Cleaves and activates cytokines such as IL36G and IL33, promoting chemokine expression and immune responses. Related references: PMID:30804664 PMID:22307629

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature form of human Cathepsin G (amino acids 21–255), which is expected to detect both pro- and mature forms of the protein.
• For Western blotting, a band around 29 kDa is predicted. Verify with a positive control such as neutrophil lysates.
• In immunohistochemistry (IHC-P), antigen retrieval methods may improve staining; validate the antibody in your tissue of interest.
• The antibody cross-reacts with mouse and rat Cathepsin G, but confirm cross-reactivity in your specific assay system before quantitative use.
• Since Cathepsin G is a secreted protease, consider using protease inhibitors in sample preparation to prevent degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of high-quality raw materials, including this Cathepsin G antibody for applications in immunology and protease research. Our supply network offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. Discover how CamelBio streamlines your assay development from concept to clinic.