CASTOR1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8WTX7

Core Product Specifications and Parameters

Biological Background: CASTOR1 Function and Localization

• Encodes cytosolic arginine sensor for mTORC1 subunit 1 (CASTOR1), also known as GATS-like protein 3 (GATSL3).
• Core component of the amino acid-sensing branch of the mTORC1 signaling pathway, functioning as an intracellular arginine sensor.
• Forms homodimers or heterodimers with CASTOR2; binds and inhibits the GATOR2 subcomplex, thereby suppressing mTORC1 activity. Binding of arginine allosterically disrupts CASTOR1-GATOR2 interaction, leading to mTORC1 activation. Related references: PMID:26972053 PMID:27487210 PMID:33594058
• Localized predominantly to the cytoplasm and cytosol.
• Widely expressed across human tissues.
• Post-translational modifications include phosphorylation (Phosphoprotein) and ubiquitination (Ubl conjugation).
• Classified under keywords: 3D-structure, Cytoplasm, Phosphoprotein, Proteomics identification, Reference proteome, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen comprises full-length human CASTOR1 (aa 1-329); for WB analysis, consider denaturing and reducing conditions to maintain epitope accessibility.
• IHC-P experiments may require antigen retrieval and tissue-specific optimization; include positive and negative controls to confirm specificity.
• In IF/ICC, confirm cytoplasmic localization; co-staining with mTORC1 pathway markers (e.g., mTOR, S6K) can provide functional context.
• ELISA protocols can be developed for CASTOR1 quantification; consider paired antibody formats for sensitive detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including antibodies targeting key signaling molecules such as CASTOR1. Our portfolio encompasses optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets, streamlining your assay development from concept to clinic. Contact us to discuss custom raw-material solutions for your mTORC1 pathway research.