Caspase-12 Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - Q6UXS9

Core Product Specifications and Parameters

Biological Background: Caspase-12 Function and Localization

• Caspase-12 (CASP12) encodes an inactive caspase that belongs to the cysteine-aspartic acid protease family but is predicted to lack catalytic activity.
• Functions as a negative regulator of inflammatory responses and innate immunity, dampening excessive immune activation (PubMed:15129283). Related references: PMID:15129283
• Suppresses cytokine release following bacterial lipopolysaccharide (LPS) stimulation, thereby limiting endotoxin-induced inflammation.
• Inhibits NF-kappa-B signaling in response to tumor necrosis factor (TNF), further modulating immune pathways (PubMed:15129283). Related references: PMID:15129283
• Exhibits broad tissue expression with highest levels in the lung, suggesting a role in pulmonary immune homeostasis.
• Listed under keywords “Alternative splicing” and “Phosphoprotein,” indicating post-translational regulation and isoform diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100), which may be conserved across species; validate sequence alignment when using mouse or rat samples.
• For Western blot, the predicted molecular weight is ~39 kDa; consider using appropriate gel percentage and loading controls.
• In IHC-P and IF-P, optimize antigen retrieval and permeabilization conditions as subcellular localization may vary.
• For ELISA, pair with a suitable detection antibody and titrate to determine optimal working concentration.
• Since caspase-12 is an inactive protease, functional assays should not rely on caspase enzymatic activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive provider of IVD raw materials and technical support services, connecting diagnostic manufacturers with high-quality reagents for research on inflammatory and immune pathways. Our portfolio includes validated monoclonal antibodies like this anti-Caspase-12 rabbit mAb, as well as antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need consistent large-scale supply or specialized raw-material procurement, CamelBio bridges the gap from concept to clinical application.