Casein Kinase 2 beta (CSNK2B) Rabbit pAb - P67870

Core Product Specifications and Parameters

Biological Background: Casein Kinase 2 beta Function and Localization

• Casein kinase II subunit beta (CSNK2B), also known as phosvitin or protein G5a, is the regulatory subunit of the serine/threonine-protein kinase casein kinase II (CK2).
• As the regulatory subunit, CSNK2B modulates the basal catalytic activity of the CK2 alpha subunit, which phosphorylates numerous substrates with acidic residues C-terminal to the target serine/threonine.
  Related references: PMID:11239457, PMID:16818610
• CSNK2B participates in the Wnt signaling pathway (by similarity).
• Upon Epstein-Barr virus (EBV) infection, CSNK2B interacts with viral EBNA1, enhancing CK2-mediated phosphorylation of PML proteins, leading to their degradation.
  Related references: PMID:20719947, PMID:24216761
• CSNK2B also suppresses EBV reactivation by mediating ARK2N and JUN at the Z promoter to inhibit BZLF1 transcription.
  Related references: PMID:31341047
• The protein localizes predominantly to the nucleus.
• CSNK2B undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination; it is associated with keywords such as zinc-binding and disease variants linked to epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human CSNK2B protein (residues 1–215), which may lead to recognition of multiple epitopes by this polyclonal antibody.
• For Western blot, expect a band at approximately 25 kDa under reducing conditions; validate using appropriate positive controls from human, mouse, or rat lysates.
• Given the nuclear localization of CSNK2B, for IHC-P and IF/ICC, optimize permeabilization and antigen retrieval steps; nuclear staining should be observed.
• The antibody is reported to cross-react with mouse and rat, making it suitable for cross-species studies; however, verify reactivity in your specific experimental context.

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