CASC5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8NG31

Core Product Specifications and Parameters

Biological Background: KNL1 (CASC5) Function and Localization

• KNL1 (also known as CASC5, AF15q14, Blinkin, hKNL-1) is a large scaffold protein and a core component of the outer kinetochore KNL1 complex.
• It serves as a docking platform for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Related references: PMID:15502821 PMID:17981135 PMID:18045986
• KNL1 directly recruits MAD2L1, BUB1, and BUB1B to kinetochores, integrating checkpoint signaling. Related references: PMID:17981135 PMID:19893618 PMID:22000412
• In coordination with CENPK, it recruits the NDC80 complex to the outer kinetochore, essential for microtubule binding. Related references: PMID:18045986 PMID:27881301
• KNL1 can bind either microtubules or protein phosphatase 1 (PP1) catalytic subunits (PPP1CA, PPP1CC), with higher affinity for PP1, suggesting regulatory roles. Related references: PMID:30100357
• The protein localizes to the nucleus, centromere/kinetochore, and cytoplasm, and is highly expressed in testis, particularly in spermatocytes and sperm acrosome.
• Associated diseases include primary microcephaly and intellectual disability; post-translational modifications include phosphorylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For western blotting, note the high molecular weight of KNL1 (265 kDa); consider using low-percentage SDS-PAGE gels and extended transfer times.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; validate staining patterns in appropriate positive control tissues (e.g., testis).
• The antibody cross-reacts with rat, but always confirm reactivity and specificity in your model system using proper controls.
• For ELISA applications, the immunogen sequence (aa 1-300) may influence assay design; consider epitope mapping if quantitative detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. For targets like KNL1 (CASC5) in kinetochore and cell division research, CamelBio can assist in sourcing rare-target antibodies and related raw materials, streamlining your development pipeline from discovery to clinic.