CAPG Rabbit Monoclonal Antibody for WB, ELISA - P40121

Core Product Specifications and Parameters

Biological Background: CAPG Function and Localization

• CAPG (Macrophage-capping protein, also known as Actin regulatory protein CAP-G, MCP, AFCP, HEL-S-66) is a calcium-sensitive actin-binding protein that reversibly caps the barbed ends of actin filaments without severing them, thereby regulating actin polymerization dynamics.
• It plays an important role in macrophage function and may also regulate cytoplasmic and nuclear structures through potential interactions with actin and DNA.
• CAPG is predominantly expressed in macrophages and macrophage-like cells, highlighting its specific role in immune cell cytoskeletal rearrangements.
• The protein localizes to multiple subcellular compartments including the nucleus, cytoplasm, melanosomes, lamellipodia, and ruffles, consistent with its involvement in cell motility and structural organization.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity in cellular signaling pathways.
• Alternative splicing yields multiple isoforms, and the protein contains repeat sequences characteristic of the gelsolin/villin family of actin regulatory proteins.
• Structural studies have resolved its 3D architecture, providing insights into its calcium-dependent actin-capping mechanism.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–188) containing actin-binding domains; therefore, the antibody may recognize both full-length and truncated CAPG forms if present.
• For Western blot, the predicted molecular weight is approximately 38 kDa; validate band specificity using positive controls such as macrophage lysates and, if available, knockout or knockdown samples.
• The antibody reacts with human, mouse, and rat CAPG; for other species, check target sequence homology before experimental use.
• In ELISA, consider using recombinant CAPG or cell lysates from macrophage lines to optimize coating and detection conditions.
• Given CAPG shuttling between nucleus and cytoplasm and its localization to membrane ruffles, subcellular fractionation or immunofluorescence may be explored with appropriate fixation and permeabilization protocols if adapting for ICC.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to validated IVD raw materials, including high-quality monoclonal antibodies like this CAPG antibody for actin cytoskeleton research. Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your workflow from concept to clinic. Leverage our expertise to accelerate your immunoassay development and biomarker discovery projects.