CAMP Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P49913

Core Product Specifications and Parameters

Biological Background: Cathelicidin Antimicrobial Peptide (CAMP) Function and Localization

• Encoded by the CAMP gene, the full-length cathelicidin antimicrobial peptide precursor (hCAP-18, ~19 kDa) undergoes proteolytic processing to yield multiple active peptides, including the well-characterized LL-37, which provides topical antimicrobial defense in sweat. Related references: PMID:14978112
• Functions as an integral component of innate immunity by binding bacterial lipopolysaccharides (LPS) and activating neutrophil N-formyl peptide receptors to promote CXCL2 release. Related references: PMID:16637646 PMID:18818205 PMID:22879591
• Exhibits broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria, including drug-resistant strains, primarily through membrane permeabilization and transmembrane pore formation. Related references: PMID:10417311 PMID:32753597 PMID:33060695 PMID:8681941
• Acts synergistically with other cathelicidin-derived peptides (e.g., KS-30, KR-31) to enhance bactericidal efficacy even under elevated salt conditions, and modulates cytokine production in keratinocytes. Related references: PMID:14978112
• Demonstrates hemolytic activity against red blood cells, reflecting its membrane‑active properties. Related references: PMID:10417311
• Primarily localized to secretory vesicles; highly expressed in neutrophilic granulocytes, bone marrow, and testis, and secreted by eccrine glands into sweat.
• Classified under keywords: Antibiotic, Antimicrobial, Innate immunity, Secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 132–170 near the C-terminus of human CAMP; the antibody may recognize both the full-length precursor (~19 kDa) and processed forms (e.g., LL-37) depending on sample context.
• For Western blotting (WB), consider using protease inhibitors during sample preparation to preserve the precursor, and validate detection of smaller cleavage products in relevant biological fluids or cell lysates.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), positive staining is anticipated in granulocytes, bone marrow, and skin eccrine glands; include appropriate controls to confirm specificity.
• For ELISA applications, pairing with a capture antibody targeting a distinct epitope may improve sensitivity when measuring processed cathelicidin peptides.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, technical support, and consulting—covering every stage from concept to clinic. This anti-CAMP antibody supports research into innate immunity and infectious disease, and is part of our extensive portfolio of validated antibodies, antigen pairs, and ancillary reagents. For bulk orders, custom antibody development, or rare-target sourcing, contact our team to streamline your diagnostic development pipeline.