CALU Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43852

Core Product Specifications and Parameters

Biological Background: Calumenin Function and Localization

• Calumenin is encoded by the CALU gene and functions as a calcium-binding protein localized primarily in the endoplasmic reticulum and Golgi apparatus, with secretion and presence in melanosomes and sarcoplasmic reticulum.
• It plays a regulatory role in vitamin K-dependent γ-carboxylation by inhibiting the gamma-carboxylase enzyme GGCX, modulating the post-translational modification of glutamate residues.
• Calumenin binds up to 7 calcium ions with low affinity, characteristic of its multiple EF-hand domains, contributing to cellular calcium homeostasis and signaling.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and glycosylation, and is subject to alternative splicing.
• Calumenin is ubiquitously expressed, with high levels in heart, placenta, and skeletal muscle; moderate levels in lung, kidney, and pancreas; and low in brain and liver, reflecting diverse physiological roles.
• It is released as a secreted protein and has been identified in melanosomes, indicating roles beyond the classical secretory pathway.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 20–315 of human CALU, which is highly conserved across species; validate specificity in your sample system.
• For western blot, a band around 37 kDa is expected; consider using a positive control such as heart or placenta lysate.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions due to the ER/Golgi localization; antigen retrieval may enhance staining.
• In ELISA, use the antibody as a capture or detection reagent after confirming performance with recombinant CALU protein.
• Cross-reactivity with mouse and rat is reported, but verify by sequence homology alignment for your target species and tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies and recombinant proteins like this CALU polyclonal antibody, supporting research in calcium signaling and carboxylation disorders. With end-to-end services from concept to clinic, we are your trusted partner for bulk reagents, custom antibody development, and rare target sourcing. Contact us to streamline your diagnostic and research workflows.