Calretinin Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P22676

Core Product Specifications and Parameters

Biological Background: Calretinin Function and Localization

• Calretinin, encoded by the CALB2 gene, is a calcium-binding protein also known as 29 kDa calbindin. It plays a critical role in calcium homeostasis and signal transduction (PubMed:2001709). Related references: PMID:2001709
• It buffers intracellular calcium levels and modulates calcium-dependent signaling pathways, thereby influencing synaptic plasticity and neuronal excitability.
• Calretinin contributes to learning and memory processes and facilitates neuronal differentiation and maturation during embryonic development.
• It is predominantly expressed in specific neuronal populations within the brain.
• Subcellularly, calretinin localizes to synapses and dendrites, consistent with its role in calcium-mediated synaptic functions.
• The protein contains calcium-binding domains and is subject to phosphorylation, as indicated by the keyword Phosphoprotein.
• Additional keywords: Metal-binding, Repeat, Cell projection, Synapse, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human Calretinin (1-271 aa), which supports detection of endogenous Calretinin in various applications.
• For Western blotting, expect a band around 32 kDa. Optimize sample preparation and loading conditions for your specific tissue or cell line.
• In immunohistochemistry (IHC) and immunofluorescence (IF), validate antibody binding in the relevant tissue or cell system. Consider antigen retrieval and permeabilization steps as needed.
• For ELISA, pair this antibody with a suitable coating antigen or secondary detection system. Confirm specificity with appropriate controls.
• Given cross-reactivity with mouse and rat, this antibody can be used in rodent models; however, always verify performance in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by offering a comprehensive portfolio of IVD raw materials, including this calretinin monoclonal antibody for neuroscience and calcium signaling studies. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your workflow. Partner with CamelBio for reliable, one-stop access to critical reagents from concept to clinic.