Calpain small subunit 1 Rabbit pAb - P04632

Core Product Specifications and Parameters

Biological Background: Calpain small subunit 1 Function and Localization

• Calpain small subunit 1 (CAPNS1) is the regulatory subunit of the calcium-dependent, non-lysosomal thiol-protease calpain.
• It mediates limited proteolysis of substrates critical for cytoskeletal remodeling and intracellular signal transduction.
• CAPNS1 is essential for embryonic development, as demonstrated by gene disruption studies (By similarity).
• The protein localizes to the cytoplasm and associates with the cell membrane, consistent with its role in membrane-proximal signaling.
• It contains calcium-binding domains, enabling calpain activation in response to intracellular calcium flux.
• Post-translational modifications include acetylation and phosphorylation, suggesting regulatory inputs beyond calcium (as indicated by proteomics data).

Experimental Guidance and Technical Tips

• Since the immunogen is a recombinant fragment (aa 69-268), the antibody may recognize both full-length and certain cleavage products. For Western blot, a band around 28 kDa is expected; consider validating specificity using recombinant protein or knockout cell lysate.
• For immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; variable cross-reactivity with rodent tissues (mouse, rat) has been noted, so pilot experiments are recommended.
• In ELISA, coating antigen should be the same recombinant protein or a peptide within the immunogen region to ensure detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. For calpain-related research and assay development, we offer bulk polyclonal antibodies like this CAPNS1 pAb, along with complementary reagents and custom sourcing services. Contact us to explore validated antibody pairs, optimized reagents, and scalable raw-material solutions for your next project.