Calmegin Rabbit mAb for WB, IF-P, ELISA - O14967

Core Product Specifications and Parameters

Biological Background: Calmegin Function and Localization

• Calmegin (CLGN) is a testis-specific molecular chaperone that belongs to the calreticulin/calnexin family of endoplasmic reticulum (ER) proteins.
• It functions during spermatogenesis by assisting the folding and quality control of client proteins essential for sperm-egg interaction, including adhesion to and penetration of the zona pellucida.
• Calmegin is required for normal sperm migration from the uterus into the oviduct and for male fertility (by similarity).
• The protein binds calcium ions, a feature critical for its chaperone activity and structural stability.
• Calmegin localizes to the endoplasmic reticulum membrane, where it mediates protein folding via its lectin-like domain.
• Post-translational modifications of Calmegin include acetylation, phosphorylation, and disulfide bond formation, which may regulate its function and interactions.
• Alternative splicing generates multiple transcript variants, potentially yielding isoforms with distinct functional properties.
• Consistent with its role in reproduction, Calmegin expression is predominantly detected in testis at the protein level.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 70 kDa; use testis-derived protein lysates as a positive control, as Calmegin expression is restricted to this tissue.
• When performing immunofluorescence (IF-P), include permeabilization steps to allow antibody access to the ER membrane-localized Calmegin; validate staining in testis sections or cell models expressing endogenous or recombinant Calmegin.
• The immunogen spans the luminal domain (aa 180–350), suggesting suitability for detecting both native and denatured forms; consider epitope accessibility in different assay conditions.
• Cross-reactivity with mouse and rat orthologs is reported; verify sequence homology and test in the appropriate species’ testis samples if used in non-human models.
• For ELISA applications, optimize coating conditions and antibody concentrations using recombinant Calmegin protein or peptide fragments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials. This Calmegin rabbit monoclonal antibody supports spermatogenesis and male fertility research, and we can further supply validated antibody pairs, bulk ancillary reagents, and rare-target raw materials tailored to your assay development needs. Partner with CamelBio for seamless procurement and technical consulting from concept to clinic.