CADPS Rabbit Polyclonal Antibody for IHC-P, ELISA - Q9ULU8

Core Product Specifications and Parameters

Biological Background: CADPS Function and Localization

• CADPS (Calcium-dependent secretion activator 1), also known as CAPS-1 or CAPS1, is a calcium-binding protein encoded by the CADPS gene (GeneID: 8618). It belongs to the CAPS family and is highly conserved across species.
• It functions primarily in the Ca²⁺-dependent exocytosis of large dense-core vesicles (DCVs) containing neurotransmitters and neuropeptides, acting at a prefusion step to facilitate vesicle-membrane fusion. It may also participate in small synaptic vesicle exocytosis.
• CADPS interacts with phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) at the membrane, and its activity is critical for catecholamine loading into DCVs and the biogenesis/maintenance of mature secretory vesicles.
• Subcellularly, CADPS localizes to the synapse, cytoplasmic vesicles, secretory vesicles, and specifically the membrane of neuronal dense core vesicles, consistent with its role in regulated secretion.
• Tissue specificity: highly expressed in neural and endocrine secretory tissues, including brain (cerebellum, cerebral cortex, medulla, occipital lobe, frontal/temporal lobes, putamen) and pancreas; lower expression in heart and fetal tissues.
• The protein undergoes alternative splicing and is phosphorylated (phosphoprotein). Its molecular weight is approximately 153 kDa (predicted 152.8 kDa), and it is annotated with keywords: calcium, lipid-binding, metal-binding, protein transport, exocytosis, and synapse.

Experimental Guidance and Technical Tips

• Since this antibody is a rabbit polyclonal raised against a recombinant fragment (aa 1020-1100), it may recognize multiple epitopes on the C-terminal region of human CADPS. For IHC-P, consider using human brain or pancreatic tissue sections as positive controls, given the high expression in these tissues.
• For ELISA applications, the immunogen fragment itself can serve as a positive control coating antigen to assess antibody titer and specificity.
• Cross-reactivity is reported for human CADPS; if working with other species, validate reactivity due to sequence homology in this region.
• The protein has a predicted molecular weight of ~153 kDa; in western blot applications (though not listed), one may observe a band around this size, but confirm the suitability of this antibody in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-CADPS pAb for neuroscience and endocrine research. We support manufacturers with optimized antibody pairs, bulk reagent supply, and custom sourcing for rare targets, streamlining the transition from discovery to diagnostics.