C8B Rabbit Polyclonal Antibody for WB, ELISA - P07358

Core Product Specifications and Parameters

Biological Background: Complement Component C8 Beta Chain Function and Localization

• Complement component C8 beta chain (C8B) is an essential subunit of the membrane attack complex (MAC), a multiprotein pore-forming complex that inserts into target cell membranes and induces cell lysis. Related references: PMID:22832194 PMID:26841837 PMID:27052168
• C8B, in conjunction with C8A and C8G, inserts into the target membrane during MAC assembly, but does not independently form pores; pore formation requires subsequent C9 polymerization. Related references: PMID:30552328
• During MAC assembly, C8B associates with C5b, C6, and C7 to form the C5b8 intermediate complex that traverses the membrane bilayer, increasing membrane rigidity. Related references: PMID:30552328 PMID:6833260
• C8B is secreted and localizes to target cell membranes upon complement activation.
• The protein contains EGF-like domains and undergoes post-translational modifications including phosphorylation and glycosylation.
• C8B functions within the innate immune system, contributing to pathogen clearance via complement-mediated cytolysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 55-350 of human C8B, covering a large portion of the protein; thus, the antibody may recognize multiple epitopes.
• For WB, a band around 67 kDa is expected; consider using appropriate positive controls (e.g., human serum or cell lines expressing C8B).
• For ELISA, validate with recombinant C8B protein or activated serum samples.
• Cross-reactivity with mouse and rat is reported; verify in your specific experimental system if using these species.
• Since C8B is secreted, sample preparation may require serum or plasma; be aware of potential interference from abundant serum proteins.
• Optimize antibody dilution empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers streamlined access to high-quality IVD antibodies and ancillary reagents, supporting diagnostic development from early-stage research to clinical validation. For complement pathway studies, we provide validated antibody pairs, monoclonal and polyclonal antibodies, and bulk raw materials tailored to your workflow. Our global supply network and technical consultation help you reliably source even rare-target reagents, ensuring continuity from concept to clinic.