c-Maf Rabbit Monoclonal Antibody for WB and ELISA - O75444

Core Product Specifications and Parameters

Biological Background: c-Maf Function and Localization

• c-Maf (Transcription factor Maf) is a member of the Maf family of basic leucine zipper transcription factors, encoded by the MAF gene (also known as proto-oncogene c-Maf).
• Functions as a transcriptional activator or repressor. In lens fiber cells, it recruits coactivators CREBBP and EP300 to crystallin promoters, driving lens-specific gene expression.
• In immune cells, c-Maf promotes Th2 differentiation by activating IL4 expression, and modulates T-cell apoptosis via interaction with MYB to suppress BCL2.
• Collaborates with PAX6 to transactivate the glucagon gene promoter and regulates CD13 promoter activity in a cell-type-specific manner (activates in endothelial cells, represses in early myelopoiesis).
• Localizes exclusively to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Reported to be expressed in endothelial cells, as per tissue specificity data.
• UniProt keywords indicate involvement in diseases such as cataract, deafness, dwarfism, and intellectual disability, and its dual role as an oncogene or tumor suppressor.
• Post-translational modifications include isopeptide bond formation and ubiquitination (Ubl conjugation), potentially affecting stability and activity.

Experimental Guidance and Technical Tips

• The immunogen peptide sequence (aa 274-373) lies within the C-terminal domain of human c-Maf; sequence homology with mouse and rat orthologs likely explains the observed cross-reactivity.
• For Western blot, the predicted molecular weight is ~38 kDa; consider using nuclear extracts to enrich the target protein and enhance detection sensitivity.
• In ELISA, ensure peptide or protein antigen coating matches the immunogen region for reliable detection; the monoclonal nature provides consistent specificity.
• Validate antibody reactivity with relevant samples, as c-Maf may be expressed at low levels in certain cell types or tissues.
• Alternative splicing variants are reported (UniProt), so confirm the detected band corresponds to the expected isoform in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to bridging IVD developers and research laboratories with high-quality raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for transcription factor and immunology research. With deep expertise in c-Maf biology—covering developmental lens disorders, Th2-mediated immunity, and oncogenic/tumor-suppressor studies—CamelBio streamlines procurement from target discovery to clinical application, ensuring a seamless supply chain for your diagnostic needs.