c-Abl (ABL1) Rabbit Monoclonal Antibody for WB, ELISA - P00519

Core Product Specifications and Parameters

Biological Background: c-Abl Function and Localization

• c-Abl (Tyrosine-protein kinase ABL1) is a widely expressed non-receptor tyrosine kinase involved in cell growth, survival, and differentiation. It localizes to the cytoplasm, cytoskeleton, nucleus, and mitochondria.
• ABL1 regulates cell adhesion and motility by phosphorylating key adaptor proteins such as BCAR1, CRK, and NEDD9. Related references: PMID:22810897
• Under oxidative stress, ABL1 translocates to mitochondria where it mediates dysfunction and cell death, partly through phosphorylation of PRKD2 at Tyr-717. Related references: PMID:28428613
• In the nucleus, ABL1 participates in DNA damage response and apoptosis by phosphorylating DNA repair factors (DDB1, RAD51, WRN) and proapoptotic proteins (TP73, CASP9).
• ABL1 regulates receptor endocytosis and autophagy: it promotes EGFR endocytosis, modulates B-cell receptor trafficking, and positively regulates lysosomal function in autophagy.
• Pathogens exploit ABL1 signaling to reorganize the host actin cytoskeleton; ABL1 phosphorylates microbial effectors like Vaccinia A36R and H. pylori CagA.
• Additionally, ABL1 positively regulates T-cell migration and homing, and controls its own activity through autocatalysis and ABI1 phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the C-terminal region (amino acids 1000–1130) of human c-Abl. This region may be present in known isoforms and fusion variants (e.g., BCR-ABL); cross-reactivity should be assessed in relevant models.
• For Western blotting, a band of approximately 123 kDa is expected. Consider using appropriate positive controls (e.g., K562 cells) and validate detection sensitivity in your sample system.
• This antibody is recommended for ELISA; optimal coating antigen concentration and detection antibodies should be empirically determined for your assay format.
• Since ABL1 is a kinase involved in multiple signaling pathways, include phosphatase inhibitors during sample preparation to preserve phosphorylation-dependent epitopes if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw materials, providing seamless access to products, technical services, and consulting from concept to clinic. This c-Abl monoclonal antibody supports oncology and cell signaling research, complementing our portfolio of validated monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For customized raw-material solutions, CamelBio solves your toughest procurement challenges.