BOB1 (POU2AF1) Rabbit Polyclonal Antibody for WB, ELISA - Q16633

Core Product Specifications and Parameters

Biological Background: BOB1 Function and Localization

• BOB1 is a transcriptional coactivator that specifically associates with POU2F1/OCT1 or POU2F2/OCT2, strongly enhancing POU2F1-mediated promoter activity and, to a lesser extent, POU2F2-mediated activity. Related references: PMID:7779176
• It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2, acting as a B-cell-specific coactivator. Related references: PMID:7859290
• BOB1 is essential for B-cell response to antigens and required for the formation of germinal centers. Related references: PMID:7623806, PMID:7859290
• It regulates IL6 expression in B cells as a coactivator of POU2F2/OCT2 (by similarity).
• BOB1 localizes to the nucleus, consistent with its role in transcription regulation.
• Tissue expression is B-cell specific; detected mainly in spleen, thymus, peripheral blood leukocyte, and small intestine. Related references: PMID:7779176, PMID:7859290
• Classified as an activator, proto-oncogene, and target of ubiquitin-like modification, BOB1 is implicated in chromosomal rearrangements and is identified by proteomics.

Experimental Guidance and Technical Tips

• The synthetic peptide immunogen spanning amino acids 150–250 has been designed to target a specific region of human BOB1; verify sequence identity in your species of interest if cross-reactivity is expected.
• For Western blot (WB), a band at approximately 27 kDa is predicted; consider using reducing conditions and a B-cell lysate (e.g., Raji or Daudi) as a positive control.
• ELISA applications can employ the immunogen peptide as a coating antigen or positive control to assess antibody binding.
• As a polyclonal antibody, lot-to-lot variability is possible; we recommend titrating the antibody and validating performance in your specific assay system.
• Additional applications may be feasible, but optimization and validation in the relevant sample matrix are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This BOB1 (POU2AF1) antibody supports research into B-cell biology and transcription regulation, making it a valuable tool for immunology and oncology studies. As a trusted IVD raw-material partner, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your diagnostic development and life science workflows.