BMPR2 Rabbit pAb for WB, IF/ICC, ELISA - Q13873

Core Product Specifications and Parameters

Biological Background: BMPR2 Function and Localization

• BMPR2 (bone morphogenetic protein receptor type-2), also known as BMP type II receptor, is a transmembrane serine/threonine kinase encoded by the BMPR2 gene (aliases: PPH1, BMR2, BMPR3, BRK-3, POVD1, T-ALK, BMPR-II).
• Upon ligand binding, it forms a heteromeric complex with type I receptors, phosphorylating and activating them, which in turn phosphorylate SMAD transcriptional regulators. BMPR2 also mediates signaling via the p38MAPK cascade. Related references: PMID:12045205
• BMPR2 binds to BMP7, BMP2, and (with lower efficiency) BMP4; binding is enhanced by type I receptors. It additionally mediates GDF6-induced adipogenesis and signals in response to activin A/INHBA. Related references: PMID:24018044
• Subcellularly, BMPR2 localizes to the cell membrane, featuring an N-terminal signal peptide, a single transmembrane helix, and an intracellular kinase domain.
• The protein contains several post‑translational modifications (disulfide bonds, glycosylation, phosphorylation) and requires divalent cations (Mg²⁺, Mn²⁺) for ATP binding and kinase activity.
• Tissue expression is highest in the heart and liver, suggesting key roles in cardiovascular and hepatic homeostasis.
• Mutations in BMPR2 are associated with primary pulmonary hypertension (PPH1), highlighting its clinical significance in vascular remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 27–150 (extracellular domain) of human BMPR2; this region may be accessible in native and denatured conditions, making the antibody suitable for applications such as WB and IF/ICC.
• For Western blotting, the predicted molecular weight of BMPR2 is ~115 kDa. Validate the antibody in the relevant sample system using appropriate positive (e.g., heart or liver lysates) and negative controls.
• In immunofluorescence/ICC, consider permeabilization protocols to allow antibody access to the intracellular domain. Co-staining with membrane markers may help confirm localization.
• ELISA performance should be validated using the recombinant immunogen or full-length protein to confirm binding specificity.
• While cross-reactivity is indicated for human, mouse, and rat, sequence differences in the target region may affect binding; always perform validation in each species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials for diagnostic manufacturers, research laboratories, and academic institutes. The BMPR2 Rabbit pAb (Q13873) supports investigations into BMP signaling pathways, pulmonary hypertension, and cardiovascular biology. To complement this antibody, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare‑target raw materials—ensuring reliable supply throughout assay development and scale‑up.