Biotin Rabbit anti-Mouse Integrin alpha 2/CD49b Monoclonal Antibody for FC - Q62469

Core Product Specifications and Parameters

Biological Background: Integrin alpha-2 Function and Localization

• Integrin alpha-2 (also known as CD49b, GPIa, DX5) is a transmembrane receptor that pairs with integrin beta-1 to form the VLA-2 complex, a key collagen receptor.
• The alpha-2/beta-1 integrin mediates platelet adhesion to collagens, and in other cells, it modulates collagen and collagenase gene expression, force generation, and extracellular matrix organization.
• This integrin also serves as a receptor for laminins, collagen C-propeptides, and E-cadherin, expanding its role in cell-matrix and cell-cell interactions.
• It is essential for early embryonic development; mice homozygous for a null mutation in the alpha-2 gene die very early in embryogenesis.
• The protein is localized to the membrane as a single-pass type I membrane protein.
• It belongs to the integrin alpha chain family, characterized by the presence of multiple FG-GAP repeats and a von Willebrand factor type A domain.
• Key structural features include N-glycosylation, disulfide bonds, and metal-binding sites for calcium and magnesium, which are critical for ligand binding and conformational regulation.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fusion protein encompassing amino acids 168-366 of mouse integrin alpha-2, a region that likely includes part of the extracellular domain. This design targets a conserved segment, which may contribute to its specificity for mouse integrin alpha-2.
• Flow cytometry (FC): This biotin-conjugated antibody is suitable for flow cytometry on mouse cells. When performing FC, use appropriate streptavidin-fluorophore conjugates for detection. Validate the staining protocol with relevant positive and negative controls, such as platelets or cells of the hematopoietic lineage known to express integrin alpha-2.
• Since integrin alpha-2 is a membrane protein, live cell staining is recommended; avoid permeabilization steps unless intracellular staining is intended. Titrate the antibody to optimize signal-to-noise ratio.
• Ensure that the biotin label does not interfere with antigen binding by comparing with unconjugated primary antibody controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with reliable access to validated antibodies, bulk ancillary reagents, and customized supply solutions. This monoclonal antibody against integrin alpha-2 expands your research capabilities in cell adhesion, platelet function, and collagen receptor biology. Through our one-stop platform, we support your procurement needs from discovery to clinical application, offering optimized antibody pairs, rare target reagents, and technical consultation without diverting your focus from core research.