Biotin Rabbit anti-Mouse IgM Monoclonal Antibody for Flow Cytometry - P01872

Core Product Specifications and Parameters

Biological Background: Immunoglobulin heavy constant mu (IgM) Function and Localization

• Immunoglobulin heavy constant mu (IgM) is the constant region of the mu heavy chain, forming the antigen-binding arms of IgM antibodies. It is encoded by the Ighm gene in the immunoglobulin heavy chain locus.
• Secreted IgM (sIgM) multimerizes predominantly into pentamers, with occasional hexamers, enabling high-avidity binding to antigens. Both natural (polyreactive) and immune (antigen-specific) sIgM are essential for effective humoral responses, mediating pathogen recognition and clearance via Fc receptors (e.g., FCMR) and complement activation (C1q classical pathway). Related references: PMID:10899913, PMID:11062505, PMID:28135254, PMID:34788614
• Membrane-bound IgM serves as the antigen receptor component of the B cell receptor (BCR) complex, providing constitutive tonic signaling for B cell survival and mediating pre-BCR signaling that regulates B cell development and allelic exclusion.
• IgM is found both secreted and on the cell membrane. sIgM is transported across mucosal epithelium via PIGR for mucosal immunity, while membrane IgM is anchored on B cells.
• Expressed specifically in B lineage cells: bone marrow B cell precursors, and mature B-1 and B-2 cells.
• Post-translational modifications include N-linked glycosylation and inter-chain disulfide bonds essential for polymerization and structural stability. Alternative splicing generates secreted and membrane-bound isoforms, and the protein contains immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail.

Experimental Guidance and Technical Tips

• This biotin-conjugated antibody detects mouse IgM in flow cytometry. Use a streptavidin-fluorophore secondary reagent for signal amplification. Consider titrating both antibody and streptavidin conjugate for optimal staining index.
• The immunogen corresponds to amino acids 105-454 of mouse IgM (constant region), enabling recognition of both secreted and membrane-bound forms. For B cell surface staining, use live, unfixed cells where possible; fixation may alter epitope accessibility.
• Include appropriate isotype controls and Fc block to minimize nonspecific binding. Cross-reactivity with other mouse immunoglobulin classes has not been reported, but validation in the relevant sample system is recommended.
• For intracellular staining of IgM, permeabilization is required; validate fixation/permeabilization protocols for your specific cell type to preserve epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-Mouse IgM monoclonal antibody as part of our comprehensive IVD raw-material portfolio for immunoassay development and B cell research. As your one-stop partner, we provide validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate diagnostics from concept to clinic. Explore our catalog for optimized reagents supporting humoral immunity and infectious disease applications.