Biotin Rabbit anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• Protein identity: CD8a (T-cell surface glycoprotein CD8 alpha chain, also known as Lyt-2) is a 27 kDa type I transmembrane glycoprotein encoded by the Cd8a gene in mouse, a key component of immune recognition.
• Coreceptor function: In T-cells, CD8a serves as a coreceptor for MHC class I:peptide complexes, binding simultaneously to the T-cell receptor (TCR) and MHC class I on antigen-presenting cells (APCs).
• Signaling mediator: CD8a recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that drive lymphokine production, motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs).
• CTL and NK roles: This mechanism enables CTLs to recognize and eliminate infected and tumor cells. In NK cells, CD8A homodimers provide a survival mechanism, facilitating conjugation and lysis of multiple target cells.
• Memory T-cell differentiation: CD8A homodimer molecules promote the survival and differentiation of activated lymphocytes into memory CD8+ T-cells.
• Post-translational modifications: The protein contains a disulfide bond, N-linked glycosylation, and palmitoylation sites, which influence membrane localization and stability.
• Subcellular localization: CD8a is localized at the cell membrane (single-pass transmembrane protein), with the extracellular domain responsible for ligand recognition.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 28–196) covers the extracellular region of mouse CD8a, indicating the antibody likely recognizes an exposed epitope suitable for flow cytometry under non-denaturing conditions.
• For FC, standard titration of the biotinylated antibody is recommended to optimize the signal-to-noise ratio; use a streptavidin-conjugated fluorophore for detection.
• Mouse CD8a is predominantly expressed on cytotoxic T-cells and a subset of NK cells; validate the antibody in relevant splenocyte or lymph node cell suspensions.
• Consider that alternative splicing may produce isoforms; confirm the recognition of your target isoform if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable, one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This biotinylated rabbit anti-mouse CD8a monoclonal antibody exemplifies our commitment to delivering high-quality immunological reagents for T-cell research and flow cytometry applications. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support sourcing of rare-target raw materials to accelerate your assay development.