Biotin Rabbit anti-Mouse CD8a Monoclonal Antibody for FC - P01731

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8a (T-cell surface glycoprotein CD8 alpha chain), also known as Lyt-2, is an integral membrane glycoprotein encoded by the Cd8a gene; alternative splicing yields multiple isoforms.
• In T-cells, CD8a acts as a coreceptor for MHC class I:peptide complexes, interacting simultaneously with the T-cell receptor (TCR) and MHC class I to recruit the Src kinase LCK, thereby initiating signaling cascades that lead to cytotoxic T-lymphocyte activation, lymphokine production, motility, and adhesion.
• CD8A homodimers on NK-cells provide a survival mechanism that facilitates conjugation and lysis of multiple target cells and also promote differentiation of activated lymphocytes into memory CD8 T-cells.
• The protein localizes to the cell membrane as a single-pass type I membrane protein, with its extracellular immunoglobulin domain mediating MHC I interaction.
• Post-translational modifications include glycosylation, disulfide bond formation, lipidation (palmitoylation), and signal peptide cleavage, as indicated by keywords Glycoprotein, Disulfide bond, Lipoprotein, and Signal.
• Key functional annotations encompass adaptive immunity, immunoglobulin domain, and transmembrane helix, consistent with its role in antigen recognition and immune cell communication.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 28–196) covers the extracellular domain of CD8a, suggesting that this antibody may recognize the native surface protein; consider its use in live-cell flow cytometry.
• For optimal performance in FC, titrate the antibody and validate with appropriate positive controls such as mouse splenocytes or CD8+ T-cell lines.
• As a biotin-conjugated reagent, detection flexibility is high; pair with streptavidin-fluorophore conjugates and optimize signal-to-noise ratios for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials. This anti-CD8a monoclonal antibody supports immune profiling applications, and we can supply complementary reagents such as matched antibody pairs, conjugated detection reagents, and ancillary bulk items to advance your assay development from concept to clinical use.