Biotin Rabbit anti-Mouse CD5 Monoclonal Antibody for Flow Cytometry - P13379

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 (Ly-1) is a lymphoid-specific cell surface glycoprotein expressed on all T-cells and the B1a subset of B-cells.
• It negatively regulates TCR signaling during thymocyte development by interacting with key signaling molecules like LCK, CD3Z, PI3K, and CBL. Related references: PMID:11157848
• CD5 mediates contact-dependent T-cell help for B-cells by binding to ligands such as CD5L and CD72, thereby promoting T-dependent B-cell activation and maintaining regulatory T/B-cell homeostasis. Related references: PMID:24950378 PMID:9064341 PMID:9723705
• It plays a critical role in thymocyte selection, T-cell effector differentiation, and immune tolerance. Related references: PMID:35720357
• In B-cells, CD5 modulates biology through ERK1/2 activation via the calcium channel TRPC1, leading to IL-10 production (By similarity).
• Subcellular localization is at the cell membrane, featuring a signal peptide, a single-pass transmembrane helix, and three scavenger receptor cysteine-rich (SRCR) extracellular domains.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, as reflected in its UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen (aa 25–371) corresponds to the extracellular region of mouse CD5, making this biotinylated antibody well-suited for surface staining in flow cytometry.
• For FC applications, use with a streptavidin-conjugated fluorophore; titrate to determine the optimal antibody dilution for your cell type.
• Include proper blocking steps to minimize non-specific binding, especially if working with cells that express high levels of endogenous biotin.
• Validate staining specificity using CD5-positive controls (e.g., mouse splenocytes or thymocytes) and negative controls (isotype control or CD5-deficient cells).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For CD5 and T-cell biology applications, CamelBio can source rare-target antibodies, conjugates, and recombinant proteins to streamline your diagnostic development. Contact us for tailored solutions from early-stage research to clinical assay manufacturing.