Biotin Rabbit anti-Mouse CD45 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 (PTPRC) Function and Localization

• CD45 (receptor-type tyrosine-protein phosphatase C) is a ubiquitously expressed leukocyte common antigen (L-CA), also known as Ly-5 and T200. Alternative splicing generates multiple isoforms, including B220 (CD45R) predominantly found on B lymphocytes.
• It contains two intracellular protein tyrosine phosphatase (PTP) domains; the first domain possesses enzymatic activity, while the second modulates substrate specificity.
• CD45 is essential for T-cell activation via the antigen receptor, serving as a positive regulator of co-activation upon binding to DPP4.
• Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN, and also dephosphorylates LYN to modulate its kinase activity.
• At antigen-presenting cell–T cell interfaces, CD45 interacts with CLEC10A on immature dendritic cells, which recognizes Tn antigen-modified CD45 on effector T cells, adjusting the activation threshold to limit autoreactivity.
• It localizes to the cell membrane, membrane rafts, and immunological synapse, consistent with its role in signal transduction.
• Post-translational modifications include heavy N-linked glycosylation and phosphorylation on serine/threonine residues.
• CD45 is a member of the protein tyrosine phosphatase family and belongs to the class I receptor-type PTPs.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the biotinylated antibody over a range of concentrations (e.g., 0.1–5 µg/test) to determine the optimal staining index with your specific cell model and secondary streptavidin conjugate.
• Include appropriate isotype control staining to assess background binding, especially when using amplified detection systems such as streptavidin-PE or streptavidin-APC.
• Prior to use, validate the antibody in the relevant mouse leukocyte population (e.g., splenocytes, lymph node cells, or peripheral blood mononuclear cells) to confirm expected expression and resolution.
• Biotin conjugation offers flexibility; consider using streptavidin conjugates matched to your instrument’s lasers and filters for single or multicolor panels.
• For indirect detection, ensure adequate wash steps after primary incubation to reduce unbound antibody and minimize non-specific signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, bridging diagnostic manufacturers, laboratories, and research institutions with a reliable supply of validated antibodies and ancillary reagents. For leukocyte biology and immunology applications, we offer one-stop access to optimized monoclonal/polyclonal antibodies, validated antibody pairs, and bulk reagents, including biotinylated detection antibodies like this CD45 clone. Leveraging our extensive supply network, we can source rare or custom targets to accelerate your assay development from concept to clinical validation.