Biotin Rabbit anti-Mouse CD45 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45 is a receptor-type tyrosine-protein phosphatase C, essential for T-cell activation via the antigen receptor.
• Possesses two intracellular phosphatase domains; the first is enzymatically active, while the second regulates substrate specificity.
• Acts as a positive co-regulator of T-cell signaling upon binding to DPP4 and dephosphorylating LYN, SKAP1, and FYN.
• Interacts with CLEC10A at the APC-T cell synapse, modulating T-cell activation threshold to limit autoreactivity.
• Localized to the cell membrane, enriched in membrane rafts and at the immunological synapse.
• Undergoes alternative splicing to generate multiple isoforms (e.g., CD45R, B220) with distinct extracellular domain compositions.
• Keywords: glycoprotein, phosphoprotein, hydrolase, transmembrane helix, repeat, synapse.
• UniProt P06800, molecular weight ~144.8 kDa.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the biotinylated antibody to determine the optimal working concentration in your experimental system, using appropriate isotype controls.
• Use with a streptavidin-fluorophore conjugate for detection; ensure the fluorophore is compatible with your cytometer configuration.
• Prepare single-cell suspensions and block Fc receptors to minimize non-specific binding.
• Since the immunogen spans the extracellular domain (aa 26-566), the antibody likely recognizes an extracellular epitope; fixation/permeabilization protocols may not be required but can be validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with reliable access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our CD45 antibody supports immunology and cell surface marker research, and we offer sourcing assistance for rare targets to streamline your development from concept to clinic.