Biotin Rabbit anti-Mouse CD4 Monoclonal Antibody for Flow Cytometry - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is an integral membrane glycoprotein and a defining marker of T-helper cells. It is also expressed in other immune cells such as macrophages, dendritic cells, and NK cells.
• Functions as a coreceptor for the T-cell receptor (TCR) by binding to MHC class II molecules on antigen-presenting cells, enhancing the sensitivity of TCR to peptide-MHC complexes.
• Simultaneously interacts with TCR and MHC class II, leading to the recruitment of the Src kinase LCK to the TCR-CD3 complex, which initiates intracellular signaling cascades essential for T-cell activation, cytokine production, and differentiation.
• Plays a critical role in thymic development of T-helper cells and drives the differentiation of monocytes into mature macrophages.
• Contains four extracellular immunoglobulin-like domains, a single transmembrane helix, and a short cytoplasmic tail with conserved motifs for LCK binding and palmitoylation.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, palmitoylation, and phosphorylation, which regulate its membrane localization and signaling functions.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 27–394 of mouse CD4, covering the extracellular domain; this design targets the native, surface-exposed epitopes, making it suitable for flow cytometry detection of live cells.
• As a biotinylated monoclonal antibody, it enables flexible detection via streptavidin-conjugated fluorophores or enzymes; titrate the antibody to determine the optimal working concentration for your specific assay.
• Validate this reagent in your mouse sample system, as cross-reactivity with other species has not been reported.
• For flow cytometry, use standard Fc receptor blocking steps to reduce background, and include appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-CD4 antibody supports immunology and T-cell biology research applications. Through our one-stop platform, we also facilitate procurement of rare-target raw materials to accelerate your diagnostic development from concept to clinic.