Biotin Rabbit anti-Mouse CD4 mAb - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4, also known as L3T4 or T4/Leu-3, is a T-cell surface glycoprotein essential for immune responses.
• It functions as a co-receptor for MHC class II molecules, interacting with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells to enhance T-cell activation.
• Upon MHC engagement, CD4 recruits the Src kinase LCK to the TCR-CD3 complex, initiating signaling cascades that promote lymphokine production, adhesion, and T-helper cell differentiation.
• Beyond T-cells, CD4 is expressed on macrophages, dendritic cells, and NK cells, where it modulates differentiation, cytokine expression, and migration in a TCR/LCK-independent manner.
• The protein is localized to the cell membrane and contains immunoglobulin-like domains, disulfide bonds, N-linked glycosylation sites, and palmitoylation and phosphorylation modifications that regulate its function.
• In thymic development, CD4 participates in T-helper cell lineage commitment and is a defining marker of mature T-helper cells, which orchestrate adaptive immunity by activating B cells, cytotoxic T-cells, and phagocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 27–394) of mouse CD4, suggesting recognition of the native surface protein; however, confirm reactivity with your target cell type in the relevant experimental system.
• As a biotin-conjugated antibody, it is compatible with streptavidin-based secondary detection in flow cytometry; titrate the antibody to optimize signal-to-noise and avoid non-specific binding.
• For FC applications, ensure appropriate compensation settings when using multiple fluorophores and include proper isotype controls to validate specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and research laboratories, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. This biotinylated anti-mouse CD4 monoclonal antibody supports immunology studies focused on T-cell biology and immune profiling. Explore our catalog or contact our team to discuss custom sourcing and technical support for your diagnostic or research pipeline.