Biotin Rabbit anti-Mouse CD25 Monoclonal Antibody for Flow Cytometry - P01590

Core Product Specifications and Parameters

Biological Background: CD25 Function and Localization

• CD25, also known as Interleukin-2 receptor subunit alpha or p55, is encoded by the Il2ra gene in mouse.
• It functions as a receptor for interleukin-2 (IL-2).
• CD25 is critically involved in immune tolerance by regulating the activity of regulatory T cells (Tregs), which suppress the activation and expansion of autoreactive T-cells.
• Subcellularly, CD25 is a membrane protein with a single transmembrane helix.
• The protein contains sushi domains, disulfide bonds, and is glycosylated.

Experimental Guidance and Technical Tips

• For flow cytometry assays, titrate the antibody to determine the optimal working concentration for your specific cell type and staining conditions.
• As a biotin-conjugated antibody, detection requires a streptavidin-fluorophore conjugate; ensure compatibility with your panel.
• Validate reactivity in the relevant mouse strain and sample type, considering potential strain-specific expression differences.
• Use appropriate isotype controls and Fc blocking to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material partner, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic and research applications. This anti-CD25 antibody supports studies in immune tolerance, Treg biology, and related immunoassay development. CamelBio streamlines procurement of rare-target raw materials, helping you accelerate from concept to clinic.