Biotin Rabbit anti-Mouse CD19 Monoclonal Antibody for Flow Cytometry - P25918

Core Product Specifications and Parameters

Biological Background: CD19 Function and Localization

• CD19 functions as a coreceptor for the B-cell antigen receptor (BCR) on B lymphocytes, lowering the activation threshold and enhancing downstream signaling. It activates pathways involving phosphatidylinositol 3-kinase and Ca2+ mobilization (PubMed:12387743, PubMed:20101619, PubMed:9382888). Related references: PMID:12387743, PMID:20101619, PMID:9382888
• While not essential for early B-cell differentiation in the bone marrow, CD19 is required for normal B-1 cell development (PubMed:7542548, PubMed:7543183, PubMed:9317126). Related references: PMID:7542548, PMID:7543183, PMID:9317126
• CD19 is critical for B-cell differentiation and proliferation upon antigen challenge, and for generating high-affinity antibodies and maintaining normal serum immunoglobulin levels (PubMed:12387743, PubMed:7542548, PubMed:7543183). Related references: PMID:12387743, PMID:7542548, PMID:7543183
• Localized at the cell membrane and enriched in membrane rafts, CD19 is expressed on B cells in spleen, bone marrow, thymus, lymph nodes, and peripheral blood lymphocytes (PubMed:12387743, PubMed:20101619, PubMed:7542548, PubMed:7543183). Related references: PMID:12387743, PMID:20101619, PMID:7542548, PMID:7543183
• CD19 contains immunoglobulin-like domains, a single-pass transmembrane segment, and carries N-linked glycosylation. It is phosphorylated upon BCR engagement, contributing to signal transduction.

Experimental Guidance and Technical Tips

• As a biotinylated antibody, pair with fluorescently labeled streptavidin conjugates (e.g., Streptavidin-PE or -APC) for flow cytometry detection; optimize the streptavidin concentration to avoid aggregation.
• Titrate the antibody over a range (e.g., 0.1–5 µg per million cells) to determine the optimal working concentration for your specific sample and instrument.
• Include appropriate isotype control antibodies and perform Fc receptor blocking (e.g., with anti-CD16/CD32) to reduce non-specific binding, especially in tissues with high myeloid cell content.
• Validate reactivity in your mouse strain of interest; CD19 expression patterns may vary slightly among common inbred strains.
• This monoclonal antibody is well suited for immunophenotyping B-cell subsets from mouse spleen, bone marrow, peripheral blood, and lymph nodes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials for immunology and B-cell studies. This anti-mouse CD19 antibody complements our range of validated antibody pairs, recombinant proteins, and ancillary reagents, all supported by one-stop sourcing and technical consultation. Contact us for bulk orders, custom conjugations, or assistance in procuring other rare targets.