Biotin Rabbit anti-Mouse CD11b Monoclonal Antibody for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: CD11b (Integrin alpha-M) Function and Localization

• Integrin alpha-M, also known as CD11b, CR3, MAC1, and CD11b/CD18, is a member of the integrin alpha chain family encoded by the Itgam gene.
• It pairs with ITGB2/CD18 to form complement receptor 3 (CR3), mediating leukocyte adhesion, phagocytosis, and migration; it binds iC3b, fibrinogen, factor X, and ICAM1.
• CD11b is predominantly expressed on the surface of monocytes, granulocytes, macrophages, and microglia, with lower expression in a subset of peritoneal mast cells.
• Subcellularly, it localizes to the plasma membrane and membrane rafts, requiring calcium and metal ions for function.
• The protein undergoes N-linked glycosylation, contains disulfide bonds, and exists in multiple isoforms due to alternative splicing.
• In the brain, CD11b/ITGAM cooperates with TYROBP/DAP12 in microglia to produce superoxide ions essential for developmental neuronal apoptosis (PubMed:18685038). Related references: PMID:18685038

Experimental Guidance and Technical Tips

• This antibody is biotin-conjugated and intended for flow cytometry; pair with streptavidin-fluorophore conjugates for detection.
• The immunogen spans amino acids 143-337 of mouse CD11b; confirm that this epitope is accessible in your experimental system.
• For optimal staining, titrate the antibody on a known positive cell line (e.g., mouse monocytes or bone marrow-derived macrophages).
• CD11b is a large glycoprotein (127 kDa); if performing intracellular staining, ensure adequate permeabilization and fixation.
• Validate cross-reactivity if used on non-mouse species, as reactivity is reported only with mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for IVD raw materials, providing everything from validated antibodies to bulk ancillary reagents. For integrin and immunology targets like CD11b, we offer high-quality monoclonals, antibody pairs, and rare-target sourcing. Reach out to discuss your specific requirements and accelerate your diagnostic development with CamelBio’s end-to-end supply chain and technical support.