Biotin Rabbit anti-Human/Monkey HLA-DR mAb for FC - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DR Alpha Chain Function and Localization

• The HLA-DRA gene encodes the alpha chain of the MHC class II molecule HLA-DR, a heterodimer with beta chain HLA-DRB, essential for presenting exogenous peptide antigens to CD4+ T cells.
• In complex with beta chain, it presents peptides typically 10-30 amino acids long, derived from endocytosed antigens processed in lysosomes, guiding T-helper effector functions. Related references: PMID:8145819
• The complex follows two processing modes: 'bind first, cut/trim later' for pathogen-derived peptides and 'cut first, bind later' for self-peptides, with a key hydrophobic anchor residue at peptide position 1. Related references: PMID:25413013, PMID:8145819
• HLA-DR is expressed on professional antigen-presenting cells including macrophages, dendritic cells, B cells, and thymic epithelial cells, as confirmed at protein level. Related references: PMID:15322540, PMID:23783831, PMID:31495665
• Subcellular localization includes cell membrane and compartments of the endocytic pathway: endoplasmic reticulum, early and late endosomes, lysosomes, and autolysosomes.
• The protein undergoes N-linked glycosylation and contains a disulfide bond; its molecular weight is approximately 29 kDa (precursor ~28.6 kDa), with additional weight due to glycosylation.
• Keywords highlight roles in adaptive immunity, host-virus interaction, and ubiquitin conjugation, and it is a reference proteome member with 3D structure available.

Experimental Guidance and Technical Tips

• This antibody targets the extracellular domain of HLA-DR alpha chain, making it suitable for live-cell flow cytometry detection of surface MHC class II molecules.
• The biotin label enables flexible detection via streptavidin conjugates; consider titrating the antibody and matching streptavidin-fluorophore for optimal signal-to-noise ratios.
• Validate antibody specificity by staining known HLA-DR-positive cells (e.g., Raji, human PBMCs) and employing appropriate isotype controls.
• For multi-color panels, confirm spectral compatibility and minimal spillover; pre-block with serum may reduce nonspecific binding to Fc receptors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This biotinylated anti-HLA-DR antibody supports MHC class II research and immune profiling applications. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.