Biotin Rabbit anti-Human/Monkey CD3 Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3 epsilon is an integral component of the TCR-CD3 complex on T lymphocytes, playing a critical role in adaptive immune responses by transmitting activation signals upon antigen recognition. Related references: PMID:15294938, PMID:15546002, PMID:2470098
• The cytoplasmic domain of CD3 epsilon contains immunoreceptor tyrosine-based activation motifs (ITAMs) that become phosphorylated by Src family kinases LCK and FYN after TCR engagement, initiating downstream signaling cascades. Related references: PMID:2470098, PMID:40592325
• CD3E is also essential for proper T-cell development and mediates internalization and surface down-regulation of the TCR-CD3 complex through endocytosis sequences in its cytosolic tail. Related references: PMID:10384095, PMID:26507128
• Apart from its coreceptor function, CD3E acts as a receptor for ITPRIPL1, inhibiting T-cell activation by promoting interaction with NCK1 and blocking ZAP70 recruitment and ERK-NFkB signaling. Related references: PMID:12110186, PMID:38614099
• The protein localizes at the cell membrane as a single-pass type I membrane protein, with an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail containing the ITAMs.
• Alternative names for CD3 epsilon include T3E, TCRE, and IMD18, and the calculated molecular weight is approximately 23 kDa.
• Post-translational modifications include phosphorylation on ITAM tyrosine residues and the presence of disulfide bonds, critical for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 1–126) of human CD3 epsilon, making the antibody suitable for detecting surface CD3 on live, non-permeabilized T cells by flow cytometry.
• Biotinylation enables flexible detection using streptavidin–fluorophore or streptavidin–enzyme conjugates, allowing for signal amplification and multicolor panel design.
• For intracellular staining or detection of total CD3E, permeabilization may be required; consider validating the antibody in your specific sample preparation protocol.
• Titrate the antibody to determine the optimal concentration that yields clear population separation while minimizing nonspecific background in your cell model and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, technical services, and consulting to support diagnostic manufacturers, labs, and research institutes from concept to clinic. This biotinylated anti-CD3 monoclonal antibody is part of our portfolio for T-cell research and immunoassay development, backed by a robust supply chain for validated antibody pairs, bulk ancillary reagents, and sourcing of rare-target raw materials. Whether you need custom conjugation, matched detection pairs, or large-scale production, CamelBio provides a flexible and collaborative path to accelerate your projects.