Biotin Rabbit anti-Human/Monkey CD3 Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3E encodes the CD3 epsilon chain, a type I transmembrane protein with a signal peptide, extracellular immunoglobulin domain, and a cytoplasmic tail containing ITAMs.
• It is a subunit of the TCR-CD3 complex on T-lymphocytes, pivotal for adaptive immunity. Related references: PMID:15294938, PMID:15546002, PMID:2470098
• Upon TCR engagement, the ITAMs are phosphorylated by LCK and FYN, initiating signaling cascades. Related references: PMID:2470098, PMID:40592325
• CD3E is essential for proper T-cell development.
• It localizes to the cell membrane and mediates internalization and down-regulation of the TCR-CD3 complex. Related references: PMID:10384095, PMID:26507128
• Additionally, it functions as a receptor for ITPRIPL1, providing an inhibitory signal by blocking ZAP70 interaction. Related references: PMID:38614099, PMID:12110186

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 1–126) of human CD3 epsilon, suggesting that the antibody likely recognizes epitopes on the cell surface, making it suitable for live-cell flow cytometry.
• This biotin-conjugated antibody can be detected with streptavidin-fluorophore conjugates, offering flexibility in multicolor panel design. Consider titrating the antibody to determine the optimal staining concentration for your specific cell type.
• Cross-reactivity with cynomolgus monkey CD3 is reported; always validate the antibody in your target species if not listed.
• For intracellular staining, perform appropriate fixation and permeabilization steps and verify compatibility with the biotin detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers by providing a comprehensive range of IVD raw materials, including validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents. For immune-oncology and T-cell research applications, we offer optimized CD3 antibodies and matching detection systems. Our one-stop service streamlines procurement from concept to clinic, ensuring reliable supply for your assay development needs.