Biotin Rabbit Anti-Human/Monkey CD20 Monoclonal Antibody for Flow Cytometry - P11836

Core Product Specifications and Parameters

Biological Background: CD20 Function and Localization

• CD20 (B-lymphocyte antigen CD20, MS4A1) is a B-lymphocyte-specific membrane protein belonging to the membrane-spanning 4-domains subfamily A. Alternative splicing generates multiple transcript variants encoding different isoforms.
• It functions as a store-operated calcium (SOC) channel component, promoting calcium influx upon B-cell receptor (BCR) activation, which is essential for B-cell development, differentiation, and activation. Related references: PMID:12920111, PMID:7684739
• CD20 plays a critical role in the regulation of cellular calcium homeostasis, mediating calcium influx necessary for B-cell signaling and function. Related references: PMID:3925015
• The protein is predominantly expressed on the surface of B-cells (cell membrane localization), serving as a specific B-cell marker and a target for immunotherapeutic antibodies.
• Post-translational modifications include palmitoylation and phosphorylation, which may regulate its membrane localization and activity.
• CD20 is a well-established pharmaceutical target, with anti-CD20 monoclonal antibodies (e.g., rituximab) used in treating B-cell malignancies and autoimmune disorders.

Experimental Guidance and Technical Tips

• This biotin-conjugated rabbit monoclonal antibody is designed for flow cytometry (FC). Optimize the antibody concentration by titration to achieve the best staining index and signal-to-noise ratio.
• Use streptavidin-conjugated fluorophores for detection. Choose a fluorophore that matches your instrument configuration and panel design.
• The antibody recognizes an epitope within amino acids 100-200 of human CD20. Validate specificity and reactivity in your sample system with appropriate controls (e.g., knockout cells or isotype controls).
• For intracellular staining, consider appropriate fixation and permeabilization protocols, as CD20 is a surface antigen.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is a trusted partner for diagnostic manufacturers and research institutes, offering a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing.
• This biotin-conjugated anti-CD20 antibody supports the development of B-cell immunophenotyping assays and diagnostics. Contact our team to discuss custom conjugation, scale-up production, and comprehensive technical consulting from concept to clinic.