Biotin Rabbit anti-Human/Monkey CD14 Monoclonal Antibody for FC - P08571

Core Product Specifications and Parameters

Biological Background: CD14 Function and Localization

• Monocyte differentiation antigen CD14, also known as My23 antigen or myeloid cell-specific leucine-rich glycoprotein, is expressed on monocytes, macrophages, and weakly on granulocytes.
• CD14 acts as a coreceptor for bacterial lipopolysaccharide (LPS), cooperating with LBP to deliver monomeric LPS to the LY96/TLR4 complex, thereby mediating innate immune responses. Related references: PMID:1698311 PMID:23264655 PMID:20133493 PMID:22265692
• Upon LPS binding, CD14 triggers MyD88/TIRAP/TRAF6-dependent signaling, leading to NF-κB activation, cytokine secretion, and inflammatory response. Related references: PMID:8612135
• CD14 also functions as a coreceptor for TLR2:TLR6 and TLR2:TLR1 heterodimers in response to diacylated and triacylated lipopeptides, respectively; these complexes signal from the cell surface and are subsequently targeted to the Golgi via a lipid-raft dependent pathway. Related references: PMID:16880211
• Binds electronegative LDL (LDL(-)) and mediates cytokine release induced by LDL(-). Related references: PMID:23880187
• Subcellular location includes cell membrane, secreted form, membrane rafts, and Golgi apparatus.
• Tissue specificity: detected on macrophages (at protein level), strongly expressed on monocytes, weakly on granulocytes, and on most tissue macrophages. Related references: PMID:1698311
• Structural features: contains leucine-rich repeats, is a GPI-anchored glycoprotein with a signal peptide, and has a predicted molecular weight of approximately 40 kDa.

Experimental Guidance and Technical Tips

• Use the biotinylated antibody for direct flow cytometry detection of surface CD14 on human or cynomolgus monkey leukocytes; ensure adequate FcR blocking to minimize nonspecific binding.
• Validate staining patterns using appropriate positive (e.g., monocytes) and negative (e.g., lymphocytes) cell populations; consider isotype controls.
• The biotin label allows flexible detection with fluorescent streptavidin conjugates; titrate the streptavidin-conjugate to optimize signal-to-noise ratio.
• For intracellular detection of CD14 in the Golgi or secretory pathway, a permeabilization step is required; note that the primary application is surface staining.
• Store the antibody under recommended cold conditions (ice bag shipping) and protect from prolonged light exposure to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a broad portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on innate immunity and inflammatory responses centered on CD14, CamelBio streamlines the sourcing of high-quality bioreagents and offers customized procurement solutions to accelerate assay development.