Biotin Rabbit anti-Human CD56/NCAM1 Monoclonal Antibody for FC - P13591

Core Product Specifications and Parameters

Biological Background: CD56/NCAM1 Function and Localization

• Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell surface glycoprotein that mediates homophilic and heterophilic cell–cell adhesion, critical for neuron–neuron adhesion, neurite fasciculation, and axonal outgrowth.
• Multiple isoforms are generated by alternative splicing, including transmembrane forms and GPI-anchored variants, as well as a secreted form; the protein contains immunoglobulin-like domains that facilitate adhesive interactions.
• NCAM1 is predominantly expressed on the surface of neurons and natural killer cells, where it plays a key role in neural development and immune surveillance, respectively.
• In addition to its canonical adhesion role, NCAM1 functions as a host cell receptor for several neurotropic viruses, including rabies virus and Zika virus, facilitating viral entry.
• The protein is extensively post-translationally modified, featuring N-linked glycosylation, disulfide bonds, and phosphorylation sites that modulate its adhesive properties and signaling.
• NCAM1 localizes primarily to the cell membrane, but can also be found as a secreted soluble form, influencing extracellular matrix interactions and paracrine signaling.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 20–413 of human NCAM1, encompassing the extracellular immunoglobulin domains; this region is exposed on the cell surface and should be accessible in live-cell flow cytometry applications.
• For flow cytometry, consider titrating the antibody to determine an optimal working concentration; include appropriate isotype controls and ensure the biotin label does not interfere with secondary detection reagents.
• Validate the antibody in the relevant sample system (e.g., human PBMCs or cultured neuronal cells) to confirm reactivity and specificity before scaling up experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated biotinylated monoclonal antibodies like this anti-CD56/NCAM1 clone, to accelerate diagnostic and life science research. We support developers with one-stop access to optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, customizing procurement solutions from concept to clinic. Explore our NCAM1-related raw materials for flow cytometry and beyond, and let us bridge your supply needs with expert technical consultation.