Biotin Rabbit anti-Human CD40 Monoclonal Antibody for FC - P25942

Core Product Specifications and Parameters

Biological Background: CD40 Function and Localization

• CD40 (TNFRSF5) is a member of the tumor necrosis factor receptor superfamily, also known as Bp50, CDw40, and CD40L receptor.
• It functions as the receptor for TNFSF5/CD40LG, transducing signals that activate ERK via TRAF6 and MAP3K8, leading to immunoglobulin secretion in B cells and macrophages. Related references: PMID:31331973
• The protein is primarily expressed on the surface of B cells and in primary carcinomas, as indicated by tissue specificity data.
• CD40 is a transmembrane glycoprotein located at the cell membrane; a secreted isoform also exists due to alternative splicing.
• It contains disulfide bonds and is glycosylated, which are characteristic post-translational modifications of its extracellular domain.
• Keywords from UniProt highlight its roles in immunity, receptor signaling, and its involvement as a disease variant.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 21-193) covers the extracellular domain, making this antibody well-suited for detecting surface CD40 in flow cytometry.
• The biotin label allows for high-sensitivity detection using streptavidin-conjugated fluorophores; consider optimizing the staining protocol for your cell type.
• This antibody is specific for human CD40; verify cross-reactivity in your sample system if using non-human species.
• For optimal performance, titrate the antibody and include appropriate controls when staining B cells or carcinoma cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw-material sourcing, technical services, and consulting—from concept to clinic. This biotin-labeled anti-CD40 monoclonal antibody supports flow cytometry-based immune profiling and disease research, aligning with CamelBio’s capability to supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare target raw-material needs or custom sourcing, our team is ready to assist you in accelerating your diagnostic development.