Biotin Rabbit anti-Human CD34 Monoclonal Antibody for Flow Cytometry - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen and transmembrane glycoprotein, also known as CD34 molecule or GIG3. The gene undergoes alternative splicing, yielding multiple isoforms.
• It functions as a possible adhesion molecule, playing a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
• CD34 may act as a scaffold for the attachment of lineage-specific glycans, enabling stem cells to bind to lectins expressed by stromal cells or other marrow components. It also presents carbohydrate ligands to selectins.
• The protein is localized to the cell membrane and contains a signal peptide and a single transmembrane helix.
• CD34 is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
• Post-translational modifications include phosphorylation and N-linked glycosylation, as indicated by proteomics data and sequence analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 170-290 of human CD34, which lies within the extracellular region (based on UniProt topology). This design supports recognition of the native surface antigen in flow cytometry applications.
• For flow cytometry experiments, consider titrating the antibody to determine the optimal concentration for your specific cell type and staining protocol. Use appropriate isotype controls and viability dyes to ensure accurate gating.
• Since CD34 is expressed on hematopoietic progenitor cells and vascular endothelium, validate the antibody in the relevant sample system (e.g., peripheral blood mononuclear cells, bone marrow aspirates, or endothelial cell lines) with known CD34 expression.
• The antibody is biotinylated; ensure your detection system is compatible with biotin-streptavidin interaction and avoid endogenous biotin interference if using tissue sections.

CamelBio: Your One-Stop Sourcing Bridge

For scientists and IVD manufacturers developing CD34-based assays for stem cell enumeration or hematopathology applications, CamelBio offers a streamlined sourcing experience. As a specialized raw-material provider, we connect diagnostic developers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and hard-to-find target raw materials—supporting your workflow from concept to clinic. Count on us for reliable, one-stop access to the critical components needed for your hematopoietic stem cell research and diagnostic development.