Biotin Rabbit anti-Human CD34 Monoclonal Antibody for Flow Cytometry - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen that functions as a possible adhesion molecule, potentially mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells.
• It presents carbohydrate ligands to selectins, facilitating stem cell homing and interaction with the microenvironment.
• The protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of various tissues.
• CD34 is a single-pass type I membrane protein with an extracellular domain, a transmembrane helix, and a signal peptide, localizing to the cell membrane.
• It undergoes alternative splicing, producing isoforms, and is subject to post-translational modifications including glycosylation and phosphorylation, which contribute to its observed molecular weight range (35-41 kDa).
• The full-length protein has a calculated molecular weight of approximately 40.7 kDa, with glycosylation often resulting in higher apparent molecular weight in SDS-PAGE.

Experimental Guidance and Technical Tips

• This biotin-conjugated antibody is suitable for flow cytometry (FC) applications, as per the supplier's recommendations; consider titrating to determine the optimal working concentration for your specific experimental system.
• The immunogen corresponds to the extracellular region (amino acids 170-290) of human CD34, suggesting the antibody can recognize native membrane-bound CD34 on live cells; ensure proper cell surface staining protocols.
• When using biotinylated antibodies, be aware of potential endogenous biotin interference, especially in tissues rich in biotin (e.g., liver, kidney); appropriate blocking steps may be required.
• Validate the antibody's performance in the relevant biological context, such as CD34-positive hematopoietic stem cells or endothelial cells, using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, offering a wide portfolio of validated antibodies, recombinant proteins, and ancillary reagents. For CD34-related research and diagnostic applications, we provide optimized monoclonal antibodies, bulk supply options, and custom sourcing for rare targets. Streamline your assay development from concept to clinic with CamelBio's one-stop technical and procurement support.