Biotin Rabbit anti-Human CD235a/Glycophorin A Monoclonal Antibody for FC - P02724

Core Product Specifications and Parameters

Biological Background: Glycophorin A Function and Localization

• Glycophorin A (GYPA), also known as MN sialoglycoprotein, PAS-2, or CD235a, is the major intrinsic membrane protein of human erythrocytes and a key determinant of the MNS blood group system.
• It is a component of the ankyrin-1 complex, contributing to erythrocyte membrane stability and shape, and is required for the high activity and plasma membrane translocation of the anion exchanger SLC4A1 (band 3).
  Related references: PMID:35835865
• The heavily glycosylated N-terminal extracellular domain carries the MN blood group antigens and serves as a receptor for Hepatitis A virus (HAV) and Plasmodium falciparum EBA-175, with the latter interaction dependent on sialic acid residues.
• As a single-pass transmembrane protein, glycophorin A localizes to the cell membrane, with its glycosylated N-terminus exposed extracellularly.
• The protein undergoes alternative splicing and post-translational modifications including phosphorylation; the calculated molecular weight of the unmodified protein is approximately 16.4 kDa, while glycosylation and processing may yield observed bands around 13–16 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20–91), making this antibody ideal for detecting cell surface glycophorin A by flow cytometry. Since the antibody is biotinylated, pair it with appropriate streptavidin conjugates for signal generation.
• For applications beyond flow cytometry, such as western blot or immunohistochemistry, careful validation is advised due to potential epitope masking by extensive glycosylation or denaturation. Consider testing under both reducing and non-reducing conditions.
• Glycophorin A expression is restricted to erythroid lineage cells; thus, human whole blood, red blood cell lysates, or erythroid cell lines are suitable positive controls, while non-erythroid cells serve as negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers with high-quality IVD raw materials, including specialized antibodies for erythroid research such as this anti-Glycophorin A monoclonal antibody. From validated antibody pairs and bulk reagents to rare target sourcing, we support every stage of your assay development—bridging the gap between concept and clinic.