Biotin Anti-Mouse CD24 Monoclonal Antibody for Flow Cytometry - P24807

Core Product Specifications and Parameters

Biological Background: CD24 Function and Localization

• CD24 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as HSA (heat stable antigen), Ly-52, and nectadrin.
• It plays a pivotal role in cell differentiation, particularly in early thymocyte development.
• Signaling is triggered by lectin-like ligand binding to CD24 carbohydrates and transduced via second messengers released from the GPI-anchor.
• CD24 modulates B-cell activation responses.
• In association with SIGLEC10, it suppresses the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Related references: PMID:19264983
• It plays a role in the control of autoimmunity. Related references: PMID:20200274
• CD24 is expressed in lymphoid, myeloid, and erythroid cells and localizes to the cell membrane.
• The mature protein has a predicted molecular weight of approximately 7.8 kDa (UniProt P24807).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 27-53), making this antibody suitable for detecting surface CD24 in flow cytometry.
• Biotinylation allows flexible detection with streptavidin conjugates; optimize streptavidin conjugate and concentration for your specific flow cytometry protocol.
• Validate the antibody in your target cell population; CD24 is expressed in lymphoid, myeloid, and erythroid lineages from mouse.
• Consider including appropriate isotype controls and blocking steps to minimize background in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and IVD developers with a comprehensive portfolio of raw materials and technical services. Our validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents streamline the transition from research to clinical application. For rare targets like CD24, our sourcing capabilities ensure reliable access to high-quality raw materials, complemented by tailored technical consulting to meet your assay development needs.