Biotin Anti-Mouse CD161c/NK1.1 Monoclonal Antibody for Flow Cytometry - P27814

Core Product Specifications and Parameters

Biological Background: CD161c/NK1.1 (Killer cell lectin-like receptor subfamily B member 1C) Function and Localization

• CD161c/NK1.1 is a stimulatory receptor on mouse natural killer (NK) cells that enhances cytotoxicity upon cross-linking, triggering calcium mobilization and interferon-gamma secretion.
• It is a type II transmembrane glycoprotein with a C-type lectin domain, localized to the plasma membrane.
• Encoded by the Klrb1c gene, it belongs to the killer cell lectin-like receptor subfamily B, and its expression is restricted to NK cells.
• The protein features disulfide bonds, N-linked glycosylation, and multiple isoforms generated by alternative splicing.
• Its molecular weight is approximately 25 kDa (calculated 25077 Da).

Experimental Guidance and Technical Tips

• As the immunogen spans amino acids 67-223 of the extracellular domain, this antibody is expected to recognize the native surface receptor, making it suitable for flow cytometry applications.
• The biotin label enables flexible detection with streptavidin conjugates, facilitating signal amplification and multiplex staining.
• For optimal performance, validate the antibody in your specific experimental system and consider using mouse NK cell lines or primary splenocytes as positive controls.
• Avoid fixation methods that may mask the epitope if performing live-cell staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting research and diagnostic development in immunology and NK cell biology. This anti-CD161c/NK1.1 antibody complements our portfolio of validated antibodies, bulk reagents, and custom development services. From antibody pairs to rare target raw materials, we streamline procurement to accelerate your concept-to-clinic journey.