Biotin Anti-Mouse CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• Mast/stem cell growth factor receptor Kit (CD117/c-Kit) is a type III transmembrane tyrosine-protein kinase encoded by the Kit proto-oncogene, serving as the receptor for the cytokine KITLG/SCF.
• Upon ligand binding, Kit activates multiple downstream signaling pathways, including PI3K/AKT, RAS/MAPK, and JAK/STAT cascades, to regulate cell survival, proliferation, and differentiation.
• Kit plays essential roles in hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and melanogenesis.
• Alternative splicing generates multiple isoforms with distinct tissue distributions: isoform 1 is widely expressed, while isoform 3 is enriched in hematopoietic stem cells.
• Subcellularly, Kit is primarily located on the cell membrane, but undergoes internalization and cytoplasmic localization following activation.
• Post-translational modifications include N-linked glycosylation and ligand-dependent phosphorylation on key tyrosine residues, which are critical for kinase activity and signal propagation.
• As a proto-oncogene, activating mutations and dysregulated expression of KIT are implicated in various malignancies, highlighting its importance as a therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 25–523) of mouse CD117/c-Kit, suggesting the antibody is suitable for detecting surface-expressed receptor in live-cell flow cytometry.
• For flow cytometry, pair this biotinylated primary antibody with a fluorophore-conjugated streptavidin secondary reagent; titrate both to achieve optimal staining index for your specific cell type.
• Include appropriate controls such as isotype-matched biotinylated antibody and, if available, cells from Kit-knockout mice or blocking with recombinant KITLG/SCF to confirm specificity.
• Consider testing cross-reactivity with human or other species if required, but note that this antibody is reported to react with mouse; validation in other species is the user's responsibility.
• While not listed, the antibody may work in other applications like immunohistochemistry or immunoprecipitation; performance should be empirically validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials. This anti-CD117/c-Kit monoclonal antibody exemplifies our commitment to supplying high-quality immunological reagents for hematology and oncology applications. From validated antibody pairs to bulk ancillary reagents, CamelBio supports your development from concept to clinic with reliable sourcing and technical expertise.