Biotin Anti-Human CD11b Monoclonal Antibody for Flow Cytometry - P11215

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M Function and Localization

• Integrin alpha-M (ITGAM, CD11b) is a type I transmembrane glycoprotein that forms the Mac-1 integrin heterodimer with the beta-2 subunit (ITGB2/CD18).
• Acts as complement receptor 3 (CR-3), binding the iC3b fragment of complement C3b to mediate phagocytosis of opsonized particles. Related references: PMID:9558116
• Mediates cell-cell adhesion through interactions with ICAM-1 on endothelial cells, supporting leukocyte extravasation. Related references: PMID:1980124
• Serves as a receptor for fibrinogen and factor X, recognizing specific peptides of fibrinogen gamma chain.
• Regulates neutrophil migration and, together with ITGB2, mediates CD177-PRTN3-dependent neutrophil activation. Related references: PMID:28807980, PMID:21193407
• Predominantly expressed on the surface of monocytes, macrophages, and granulocytes, with key roles in innate immunity.
• Localizes to the plasma membrane and membrane rafts, requiring divalent cations (Ca²⁺, Mg²⁺) for ligand binding.
• The ITGAM gene is linked to systemic lupus erythematosus susceptibility (SLEB6).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human CD11b; consider using a peptide competition assay to confirm binding specificity in your experimental system.
• For flow cytometry, titrate the antibody to determine optimal working dilution and include appropriate isotype controls to minimize non-specific staining.
• Since CD11b is expressed on the extracellular surface of leukocytes, perform surface staining on live cells; if intracellular detection is needed, optimize fixation and permeabilization conditions as this application has not been validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B partner for diagnostic manufacturers and research institutions, offering a comprehensive portfolio of IVD raw materials. This biotin anti-human CD11b antibody supports flow cytometry-based immunophenotyping and complement receptor studies. Beyond this reagent, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your diagnostic development pipeline.