Biotin Anti-HLA-DR Monoclonal Antibody for Flow Cytometry - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DR Alpha Chain Function and Localization

• HLA-DRA encodes the alpha chain of MHC class II molecules, which pairs with the beta chain (HLA-DRB) to form functional HLA-DR heterodimers on the cell surface.
• In complex with HLA-DRB, presents antigenic peptides on professional antigen-presenting cells (APCs) for recognition by CD4+ T cells, guiding T-helper effector functions such as antibody-mediated immune response and macrophage activation. Related references: PMID:15265931, PMID:15322540, PMID:17334368
• Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. Related references: PMID:8145819
• In the tumor microenvironment, presents antigenic peptides primarily generated via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Related references: PMID:31495665
• Presents peptides derived from intracellular proteins trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. Related references: PMID:17182262, PMID:23783831
• The selection of immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived peptides and 'cut first, bind later' for autoantigens/self-peptides, with a large hydrophobic anchor residue at peptide position 1 essential for high-affinity interaction with MHCII. Related references: PMID:25413013, PMID:8145819
• Localizes to the cell membrane and to membranes of endocytic compartments including early endosomes, late endosomes, lysosomes, and autolysosomes, consistent with its role in antigen processing and presentation.
• Expressed in professional APCs such as macrophages, dendritic cells, and B cells, as well as in thymic epithelial cells.

Experimental Guidance and Technical Tips

• This biotin-conjugated monoclonal antibody is suitable for direct flow cytometry detection of HLA-DR on human and cynomolgus APCs without the need for a primary-secondary antibody pair.
• Validate the antibody in your specific sample system, as HLA-DR expression levels may vary depending on cell type, activation state, and disease context.
• For multicolor flow cytometry, ensure compatibility of the streptavidin-conjugated fluorophore with other fluorophores in your panel to avoid spectral overlap.
• Because the immunogen is a recombinant protein, the antibody may target a conformational epitope; consider this if using denaturing techniques (e.g., Western blotting under reducing conditions).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw-material procurement, providing diagnostic manufacturers and researchers with validated antibodies, bulk ancillary reagents, and tailored raw-material solutions. This anti-HLA-DR antibody exemplifies our offering in immune monitoring and MHC class II research. Whether you need antibody pairs, optimized monoclonal antibodies, or rare target sourcing, CamelBio streamlines your supply chain from concept to clinic.